生物芯片技术及其应用 南京医科大学第一附属医院 临床检验中心 童明庆
生物芯片技术及其应用 南京医科大学第一附属医院 临床检验中心 童明庆
1.What is a microarray Microarray或Biochip(生物芯片)是 种微型多参数生物传感器。它通过在一微小 的基片表面固定大量的分子识别探针，或构建 微分析单元和系统，实现对化合物、蛋白质、 核酸、细胞或其它生物组分准确、快速、大信 息量的筛选或检测。 A microarray is an ordered array of microscopic elements on a planar substrate that allows the specific binding of genes or gene products
1.What is a microarray • Microarray 或 Biochip （生物芯片）是 一种微型多参数生物传感器。它通过在一微小 的基片表面固定大量的分子识别探针，或构建 微分析单元和系统，实现对化合物、蛋白质、 核酸、细胞或其它生物组分准确、快速、大信 息量的筛选或检测。 • A microarray is an ordered array of microscopic elements on a planar substrate that allows the specific binding of genes or gene products
1.What is a microarray Four distinct characteristics of microarray Microscopic target elements or spots; 。Planar substrates, Rows and columns of elements; Specific binding between microarray target element on the substrate and probe molecules in solution
1.What is a microarray Four distinct characteristics of microarray : • Microscopic target elements or spots; • Planar substrates; • Rows and columns of elements; • Specific binding between microarray target element on the substrate and probe molecules in solution
1.What is a microarray Which is the probe?and which is the target? There are some differences between China and United States of America. In U.S.A.the probe are usually in solution. In China the probe is a reagent to detect target from sample
1.What is a microarray Which is the probe?and which is the target? There are some differences between China and United States of America. In U.S.A. the probe are usually in solution. In China the probe is a reagent to detect target from sample
Biochips的主要类别 蛋白芯片Protein chips(protein microarray) 。 肽芯片，蛋白芯片 基因芯片Gene chips(Gene microarray): 靶DNA芯片，多探针芯片 ③芯片实验室Laboratory-on-a-chip or Microfluidics-based chip
Biochips的主要类别 蛋白芯片 Protein chips(protein microarray)： 肽芯片，蛋白芯片 基因芯片 Gene chips（Gene microarray）： 靶DNA芯片，多探针芯片 ③芯片实验室Laboratory-on-a-chip or Microfluidics-based chip