Antibodies Introductory article Moncef National de Recherche et de Snt Medicale,Pris,France Artice Contents Introduction Antigensareantigen-reactive proteins,esignated,presentinthe Antibody Classes and Subdasses plasma and in extracellular fluids.They bind their nominal antigens with exquisite Cellular Origin specificity and potentially neutralize their harmful effects. Functio Introduction Antibodies were the first elements ofthe immune system to beidentiel.TheyareantienrCactreprotcinspregen shared by all individuals of a given species m and obtained In hum ns,five classes of immunoglobulins,also called d sotypes (IgG.IgM.IgA.IgD and IgE).differ in thei immunogen.By contrast.normal serum.also called of the preimmune serum,does not contain antibodies specific s (Table 1).In for that ani their monomeric form.all classes exhibit the same basic As the be structure:two heavy (H)and two light(L)chains.While lobun serum prote IgD an gE consist of a single monomeric unit, Characteristically,antibodies,which are found in the bind the nomina Formation of these polymers requires an additional subdivision In addition to the five major classes,there are four subclasses of IgG in humans Antibody Classes and Subclasses (IgGl to IgG4)and in mice (IgGl,IgG2a,IgG2b and g).and two subclasses of igA in humans l and it the omic level the human H-chain idiotopes reflect the uniqueness ofstructural features of the adpeminapeicoranindiid ind s.Each H-chain locus codes for a constant region o ay mo the vana e regions b allelic polymorp Table 1 Characteristics of human immunoglobulin isotypes IgGI IgG2 IgG3 IgG4 IgM IgAl IgA2 IgD IgE Heavy chain e No.of constant domains 3 Light chain ,Kor Kor入Kor入Kor Kor Kor入Kor入Kor Molecular weight(kDa) 150 150 165 150 970 160 160 175 190 Serum concentration(mg mL-1) 9 3 05 1.5 3 0.5 0.04 0.0003 Half-life (davs) 23 23 7 23 5 6 6 3 2.5 Placental transfer ++++ +十++++ Binding to macrophages and other phagocytic cells + + 、 Binding to basophils and mast cells Comple (classical pathway + Complement activation(alternate pathway) ENCYCLOPEDIA OF LIFE SCIENCES/2001 Nature Publishing Group /www.els.net
Antibodies Moncef Zouali, Institut National de la Recherche et de la Sante´ Me´dicale, Paris, France Antigens are antigen-reactive proteins, designated immunoglobulins, present in the plasma and in extracellular fluids. They bind their nominal antigens with exquisite specificity and potentially neutralize their harmful effects. Introduction Antibodies were the first elements of the immune system to be identified. They are antigen-reactive proteins present in an immune serum, called antiserum, and obtained after exposure of the vertebrate host to a given antigen, called an immunogen. By contrast, normal serum, also called preimmune serum, does not contain antibodies specific for that antigen. As the overwhelming majority of antibody activity proved to be associated with the gglobulin peak seen in fractionation of serum proteins, immune globulins were designated immunoglobulins. Characteristically, antibodies, which are found in the plasma and in extracellular fluids, bind their nominal antigens with exquisite specificity and potentially neutralize their harmful effects. Antibody Classes and Subclasses Serological studies have led to the description of three important determinants on the antibody molecule. First, idiotopes reflect the uniqueness of structural features of the variable region determinants specific for an individual antibody, or idiotype. Second, allotopes are antibody characteristics reflecting allelic polymorphisms that differ between individuals of the same species. Third, isotopes designate antigenic determinants present on antibodies shared by all individuals of a given species. In humans, five classes of immunoglobulins, also called isotypes (IgG, IgM, IgA, IgD and IgE), differ in their physicochemical and serological properties, and in the amino acid sequence of their constant regions (Table 1). In their monomeric form, all classes exhibit the same basic structure: two heavy (H) and two light (L) chains. While IgG, IgD and IgE consist of a single monomeric unit, the IgM and IgA classes are composed of monomers that associate to form pentamers and dimers, respectively. Formation of these polymers requires an additional polypeptide chain termed the J chain. Serological analysis of immunoglobulins of the same class revealed the existence of additional heterogeneity and resulted in the subdivision of classes into subclasses. In addition to the five major classes, there are four subclasses of IgG in humans (IgG1 to IgG4) and in mice (IgG1, IgG2a, IgG2b and IgG3), and two subclasses of IgA in humans (IgA1 and IgA2). At the genomic level, the human H-chain isotypes are encoded by nine constant loci: m, d, g1, g2, g3, g4, a1, a2 and e. Each H-chain locus codes for a constant region of the antibody molecule, the variable regions being encoded for by a distinct, large library of gene segments. Although Article Contents Introductory article . Introduction . Antibody Classes and Subclasses . Cellular Origin . Structure . Function Table 1 Characteristics of human immunoglobulin isotypes IgG1 IgG2 IgG3 IgG4 IgM IgA1 IgA2 IgD IgE Heavy chain γ1 γ2 γ3 γ4 µ α1 α2 δ ε No. of constant domains 3 3 3 3 4 3 3 3 4 Light chain κ or λ κ or λ κ or λ κ or λ κ or λ κ or λ κ or λ κ or λ κ or λ Molecular weight (kDa) 150 150 165 150 970 160 160 175 190 Serum concentration (mg mL–1) 9 3 1 0.5 1.5 3 0.5 0.04 0.0003 Half-life (days) 23 23 7 23 5 6 6 3 2.5 Placental transfer +++ + +++ +++ – – – – – Binding to macrophages and other phagocytic cells + – – + – – – – + Binding to basophils and mast cells – – – – – – – – + Complement activation (classical pathway) ++ + +++ – +++ – – – – Complement activation (alternate pathway) – – + – – + – – – ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net 1
Antibodies the different subclasses belonging to the same class are tion.Once a b lvmphocyte is committed to antibod encoded by different exons,they show greater sequence secretion,the combination of its variable region genes will oeloeheireonantregorsandehibtacommoa comp cis (humans15:mice=19).In humans,there is one will be assembled to variable region genes.so that the firs Ck locus and four C loci (CI to CA4),which comprise isotype to beexpressed at the B-lymphocyte surface is IgM four A subclasses. the H-chain Even though membrane-bound and secreted IgM have th to the antibody molecule.and each antibody isotype has u chain that is 21 amino acids longer than that of the secreted form;most of the extra residu s are uncharged and mmune systen ch e o m are h drophobic,enabling anchorage of the ne le binding al Fe ulation.B cells proliferate,differenti facilitates transport of maternal IgG into the fetus.On the ate and may recombine another constant exon(Cy.Ca or other hand.antibodies of the IgE isotype bind to specific Ce).giving rise to IgG,IgAor IgE antibodies.This process, called sotype switching or h g city to the property,IgE antibodies play an important role in allergic function.It is remarkable that antibodies of different disorders and parasitic infection Consequently, the variable region isotypi antibody response Isotype switching usu econdar thtprto to the iing by immunization or following infection.Different cytokines functionierenta to unc ons,an m response g s rise in all and IgG2 antibody production is triggered in response to there is no class switching for the Lchain. carbohydrate antigens. With successive exposures to the antigen,the isotype and the magnitude Cellular Origin The human bod Tand B ohcooiiciphetamantpeaid he imm h the h response to antgen)use f the r bone marrow,migrate to secondary lymphoid organs decline progressively.A second exposure to the same (spleen,lymph nodes.Peye tonsi appendix) namn led boostin anc fron ized by the uniqueness of its variable regions and the pairing of its H and L chains.This Persistence of memory lymphocytes for many years combinatorial diversity generates a repertoire of lympho provides the ecipient with a pe tential long-lasting protec- cytes bea ainst the inva It is this immunologic memory the B-cell clone is activated and divides rapidly.thus ondary expanding the antigen-specific clone. higher affinity for the immunogen than the primary IgM. ocyte switched cell will evolve to a plasma cellor toa into ant ng a unique imn in recep of the same antigen-bindin ence of T cells and usually involves both memc ry R the lymphocyte progeny after cell activation and prolifera cells and memory Tcells.This increase in affinity enhances 2 ENCYCLOPEDIA OF LIFE SCIENCES/62001 N els net
the different subclasses belonging to the same class are encoded by different exons, they show greater sequence homology in their constant regions and exhibit a common overall molecular structure. The L chain comprises two isotypes (k and l) and the k/l ratio varies in different species (humans 5 1.5; mice 5 19). In humans, there is one Ck locus and four Cl loci (Cl1 to Cl4), which comprise four l subclasses. At the functional level, differences in the H-chain constant regions of the isotypes confer distinct properties to the antibody molecule, and each antibody isotype has been adapted to function in a different compartment of the immune system. It is the H-chain isotype that determines the locations of the antibody and its half-life in vivo. For example, binding of IgG isotypes to placental Fc receptors facilitates transport of maternal IgG into the fetus. On the other hand, antibodies of the IgE isotype bind to specific receptors on mast cells and basophils and, upon crosslinking by antigen, induce release of mediators and cytokine production by these cells. Because of this unique property, IgE antibodies play an important role in allergic disorders and parasitic infections. Consequently, the isotypic composition of an antibody response imparts the tissue distribution of the immunoglobulins produced and their biological functions, each isotype contributing distinctively to protection against pathogens. IgG subclasses, for example, exhibit distinct structures and effector functions, and are differentially expressed in response to specific stimuli. In humans, IgG1 predominates in antiviral responses; specific IgG4 levels rise in allergic responses; and IgG2 antibody production is triggered in response to carbohydrate antigens. Cellular Origin The human body contains approximately 2 1012 T and B lymphocytes. Unlike T lymphocytes, which must migrate through the thymus, B lymphocytes originate from the bone marrow, migrate to secondary lymphoid organs (spleen, lymph nodes, Peyer patches, tonsils, appendix) and produce immunoglobulins. Each B lymphocyte, or Bcell clone, is committed to the synthesis of one antibody specificity characterized by the uniqueness of its variable regions and the pairing of its H and L chains. This combinatorial diversity generates a repertoire of lymphocytes bearing receptors that can recognize virtually all possible antigens. Upon interaction with its target antigen, the B-cell clone is activated and divides rapidly, thus expanding the antigen-specific clone. The progeny of the stimulated B lymphocyte eventually differentiate into antibody-secreting cells, called plasma cells. The membrane of the B lymphocyte bears antibodies of the same antigen-binding specificity as those secreted by the lymphocyte progeny after cell activation and proliferation. Once a B lymphocyte is committed to antibody secretion, the combination of its variable region genes will be expressed by all members of the progeny. At early stages of B-cell development, it is the Cm exon, which lies closest to the immunoglobulin H-chain variable region locus, that will be assembled to variable region genes, so that the first isotype to be expressed at the B-lymphocyte surface is IgM. Even though membrane-bound and secreted IgM have the same specificity, the two forms produced by the cell clone are not completely identical. Membrane-bound IgM has a m chain that is 21 amino acids longer than that of the secreted form; most of the extra residues are uncharged and some of them are hydrophobic, enabling anchorage of the m chain in the membrane. With antigen stimulation, B cells proliferate, differentiate and may recombine another constant exon (Cg, Ca or Ce), giving rise to IgG, IgA or IgE antibodies. This process, called isotype switching or class switching, enables antibodies of a given specificity to acquire a different constant region and, hence, to change their effector function. It is remarkable that antibodies of different isotypes can express exactly the same variable region. Isotype switching usually takes place during the secondary immune response, i.e. following several rounds of exposure of the antigen to the immune system by deliberate immunization or following infection. Different cytokines preferentially induce switching to different isotypes by making the switch recombination sites that lie 5’ to each Hchain constant exon accessible to switch recombinases. As the k and l exons are located on different chromosomes, there is no class switching for the L chain. With successive exposures to the antigen, the isotype and the magnitude and affinity of the antibody response change. Following initial antigen injection, termed priming, the recipient mounts a primary immune response, which is influenced by the nature of the antigen, the dose injected, the route of injection, the adjuvant (a preparation that enhances the immune response to an antigen) used and the genetic make-up of the immunized host. After reaching a plateau, the amount of the primary IgM produced will decline progressively. A second exposure to the same antigen, called boosting, triggers a secondary immune (anamnestic) response that differs in several respects from the primary response. It is produced more rapidly, its magnitude is higher, and it persists for longer time periods. Persistence of memory lymphocytes for many years provides the recipient with a potential long-lasting protection against the invader. It is this immunological memory that allows successful vaccination and prevention against pathogens. Secondary antibodies are dominated by IgG of higher affinity for the immunogen than the primary IgM. The isotype-switched cell will evolve to a plasma cell or to a memory cell expressing a unique immunoglobulin receptor. The secondary immune response depends on the presence of T cells and usually involves both memory B cells and memory T cells. This increase in affinity enhances Antibodies 2 ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net
Antibodies agains e at part erial tox nde den are the lymph nodes and the spleen. where antiger ucture (pneu ibility comple (MHC)for to T play an im The a e against bacterial Activation of B lymphocytes requires antigen recognition pathogens with cell wall polysaccharides. through the B-c cell receptor.but also a second signal ered by T een T and Structure blasts and begin to divide.leading to formation ofgerminal centres.When they recogniz helper cells Each millilitre of normal serum contains approximately B o mmunoglo ules.Even anti odes specif eted antibodies ted via lym phatic ised aga ontains many diffe antibodies which bind to the antigen in slightly different associated some of them ct with related re even able of stimulating an IgA response.The bulk of IgA found in the requires use of homogeneous antibodies produced either secretions roduce by by neoplastic plasma cells,termed myeloma cells,or by plasm ng in a propria pre hybridoma cells erate and It is a polymeric form of lA containing an additional ed to as my teins component M conta ng a myeloma cell line to ound or an The rom a basolateral membrane recentor for I chain containins only one kind of antibody.referred to as a monoclonal polymeric immunoglobulins,referred to as the polymeric During tran Basically.the immu molecule is a Y-shap o. po to the menic protein c of two release of slgA The rest of the IgA produced at the mucosal sites is released to the systemic circulation where it mmunoglobulins with enzymes and chemical reagents will oioutenoeher apable of cl eaving peptide bonds breaks them up into ating Ig secondary agents that cleave disulfide bonds generate two H-chain surface of mucosal tissues sic molecule into th cytes,the called helper T cell indenendent can tri an antibody eferred to as Fab fragments (for 'fragn ent antiger response in the absence of T lymphocytes.Antigens of the binding).Each fragment possesses only one combining nt me precipi ate,the antigen.The nt er ependent type agment ess called polyclonal Fefragment (forfragment crystalline).Finally,treatment activation.However,they cannot induce isotype switch with pepsin gives rise to several small fragments and a large ragle tha the ial lipopolysa F(abb ENCYCLOPEDIA OF LIFE SCIENCES/2001 Nature Publishing Group /www.els.net
the protective effect of antibodies present at low concentrations, particularly those directed to soluble antigens active at low concentrations, such as bacterial toxins. The major sites of formation of high-affinity antibodies are the lymph nodes and the spleen, where antigenpresenting cells degrade the protein antigen into peptides that bind to class II molecules of the major histocompatibility complex (MHC) for presentation to T cells. Activation of B lymphocytes requires antigen recognition through the B-cell receptor, but also a second signal delivered by T cells. Most of the interaction between T and B cells occurs in the primary follicle of secondary lymphoid organs. After cell interaction, lymphocytes transform into blasts and begin to divide, leading to formation of germinal centres. When they recognize antigen, helper T cells activate B cells to proliferate and differentiate into antibody-producing cells and memory lymphocytes. Secreted antibodies are transported via efferent lymphatics into the venous system and circulate through the peripheral blood to accomplish their effector functions. Some antigens are able to reach the mucosa-associated lymphoid tissue (present in the gut, lung, mammary, lachrymal and salivary glands) in a form capable of stimulating an IgA response. The bulk of IgA found in the secretions, called secretory IgA (sIgA), is produced by plasma cells residing in the lamina propria. It predominates in exocrine secretions of humans and most mammals. It is a polymeric form of IgA containing an additional glycoprotein chain, designated ‘secretory component (SC)’. Secretory IgM (sIgM), a form of IgM containing bound SC, also occurs in secretions. The SC is synthesized in epithelial and glandular cells. It is first expressed as a basolateral membrane receptor for J chain-containing polymeric immunoglobulins, referred to as the polymeric immunoglobulin receptor (pIgR). During transepithelial transport, polymeric IgA is disulfide bonded to the SC, which is cleaved from its transmembrane segment before release of sIgA. The rest of the IgA produced at the mucosal sites is released to the systemic circulation where it will constitute, together with IgA produced in secondary lymphoid organs, systemic IgA. Circulating IgA does not contain SC, but may occur in both monomeric and J-chain polymeric forms. IgA antibodies neutralize toxins at the surface of mucosal tissues. Because antibody production to some antigens requires the help of T lymphocytes, these antigens are called helper T cell dependent. However, a second group of antigens, called helper T cell independent, can trigger an antibody response in the absence of T lymphocytes. Antigens of the second group activate B cells by different mechanisms and fall into two classes. Helper T cell-independent type 1 antigens can induce proliferation and differentiation of immature and mature B cells, a process called polyclonal activation. However, they cannot induce isotype switching, affinity maturation and generation of memory B cells. The prototype of these antigens are bacterial lipopolysaccharides, which play an important protective role against extracellular pathogens. The second class, termed helper T cell-independent type 2 antigens, can activate only mature B cells. The response is dominated by a subset of B cells bearing the CD5 marker. They generally comprise a repetitive structure (pneumococcal polysaccharide, Salmonella-polymerized flagellin). The antibodies produced play an important early protective role against bacterial pathogens with cell wall polysaccharides. Structure Each millilitre of normal serum contains approximately 1016 immunoglobulin molecules. Even antibodies specific for the same epitope are heterogeneous. An antiserum raised against a given immunogen contains many different antibodies which bind to the antigen in slightly different fashions, and some of them may cross-react with related antigens and even with antigens exhibiting no obvious structural similarity with the immunogen. Because of this heterogeneity, study of the immunoglobulin structure requires use of homogeneous antibodies produced either by neoplastic plasma cells, termed myeloma cells, or by hybridoma cells. Myeloma cells proliferate and secrete immunoglobulins indefinitely and their products are referred to as myeloma proteins, found in the serum of diseased subjects and animals. Hybridoma cells are obtained experimentally by fusing a myeloma cell line to a normal B lymphocyte obtained from a human or an animal. The hybridoma grows continuously and secretes only one kind of antibody, referred to as a monoclonal antibody. Basically, the immunoglobulin molecule is a Y-shaped tetrameric protein characteristically composed of two H and two L polypeptide chains held together by covalent (disulfide) and noncovalent bonds (Figure 1). Treatment of immunoglobulins with enzymes and chemical reagents capable of cleaving peptide bonds breaks them up into fragments. Three types of treatment are particularly important in understanding antibody structure. First, agents that cleave disulfide bonds generate two H-chain polypeptides of approximately 50 kDa and two L chains of 25 kDa. Second, papain splits the basic molecule into three fragments of approximately 45 kDa. Two of them retain the antibody ability to recognize the antigen and are referred to as Fab fragments (for ‘fragment antigen binding’). Each fragment possesses only one combining site and can bind, but cannot precipitate, the antigen. The third fragment produced by papain digestion can be crystallized from a solution and is therefore referred to as Fc fragment (for ‘fragment crystalline’). Finally, treatment with pepsin gives rise to several small fragments and a large fragment, the F(ab)’2 fragment, of molecular weight double that of one Fab fragment, and capable of binding Antibodies ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net 3
F(aby2 composed of two lavers of polypeptide chains linked by a disulfide intrachain bridge in the centre of the domain.The domain.For the H chain.the v.8 and a chains consist of one variable (VH)and three constant (Cul to CH3) enghnCr.CSndEcha domains,an a fr units.are almost always pr sent.Simple or branched,the immunoglob udicd.The quence similarity among various domains sug sts that they are phylogenetically related,having evoved from a nd Fc of the in noglobulir is a sh. This proline- nonglobu porti on o olypeptid other This fe N-linked sugar antigen binding and effector functions.The cysteine -5-5-Disulfide bond Diagramof ins together. ween the Hand L chain Iso er in the numb t ( nt c ocation of interchain disulfide bonds and in the number of oligosaccharide moieties attached to the H chain.In of dditio nd onstant At the amino acid level,the variable region is comprised t and it is cap of binding and prec ating the three ne variab ilty which includ rontact the virtue of m and precipit ating the The Fab the antigen- mining or CDRs.Intersp ong the CDR are framework residues that represent approximately80% receptors of various effector cells. of the variable region ess varable and In humans,both k andchains of the Lchain consist of D pproximately amino acid residues By contras the izes P 446n dues for th the antigenic determinant(also called an epitope).Because 1 chai of the the u chain.This length variability is due essentially to the complexity of antigens each nolecule rent th ns med continu or linea Othe Each antibody chain is discontinuous or conformational epitopes,involve sites nd a variable region.In addition,comparison of antibody distributed in dif erent parts of the molecule,but brought amino acid sequences has revealed thre the existence appro rystals have revealed that the constant regions of the H amino a tr ur and L chains do not contribute to formation of the ENCYCLOPEDIA OF LIFE SCIENCES/62001 Na
and precipitating the antigen. The Fab is the antigenbinding constituent and the Fc fragment is the anchoring site for proteins of the complement system and for receptors of various effector cells. In humans, both k and l chains of the L chain consist of approximately 250 amino acid residues. By contrast, the different types of H chain vary considerably in length, from 446 amino acid residues for the g1 chain to 550 residues for the m chain. This length variability is due essentially to the presence of additional residues at the C-terminal end of the m and a chains. These extra amino acids participate in polymerization of immunoglobulins. Each antibody chain is composed of a constant and a variable region. In addition, comparison of antibody amino acid sequences has revealed the existence of homology regions, or domains, each of approximately 110 amino acids. Each domain is a globular structure composed of two layers of polypeptide chains linked by a disulfide intrachain bridge in the centre of the domain. The chains exhibit a b-strand conformation and form two antiparallel b sheets that pack together. An L chain is composed of one variable (VL) and one constant (CL) domain. For the H chain, the g, d and a chains consist of one variable (VH) and three constant (CH1 to CH3) domains, and the m and e chains exhibit an extra constant domain (CH4). Carbohydrate chains of variable shape and length, ranging from two sugar residues to a dozen or more units, are almost always present. Simple or branched, the carbohydrate chains normally attach to the Fc portion (the CH2 domain) of the immunoglobulin. This domain organization appears to be an important feature shared by immunoglobulins from all species studied. The sequence similarity among various domains suggests that they are phylogenetically related, having evolved from a single ancestral gene. Linking the Fab and Fc regions of the immunoglobulin is a short segment of the H chain between the CH1 and CH2 domains, known as the ‘hinge’, with no sequence similarity to any of the other domains. This proline- and cysteinerich, nonglobular portion of the polypeptide backbone allows segmental flexibility of the Fab arm and Fc region relative to one another. This flexibility is important for antigen binding and effector functions. The cysteine residues form interchain disulfide bonds linking the two H chains together. In addition to these interchain bridges, most immunoglobulins comprise a disulfide bridge between the H and L chain. Isotypes differ in the number and location of interchain disulfide bonds and in the number of oligosaccharide moieties attached to the H chain. In addition, the m and e chains contain an additional constant region domain that replaces the hinge region found in the g, d and a chains. At the amino acid level, the variable region is comprised of three regions of extreme variability which include residues that may contact the antigen by virtue of mutual complementarity. They are called complementarity-determining regions, or CDRs. Interspersed among the CDRs are framework residues that represent approximately 80% of the variable region and are less variable and more evolutionarily conserved. At the three-dimensional level, the three CDRs of each chain converge to form a combining site (also called a paratope) which recognizes the antigenic determinant (also called an epitope). Because of the complexity of macromolecular antigens, each molecule has many different epitopes. Some of them are composed of a single segment of the molecule and are termed continuous or linear epitopes. Others, called discontinuous or conformational epitopes, involve sites distributed in different parts of the molecule, but brought together in the three-dimensional structure. X-ray diffraction crystallographic studies of antibody crystals have revealed that the constant regions of the H and L chains do not contribute to formation of the S S Fab -S-SFc CDRs S S -S-SCH1 VH CL VL N-linked sugar -S-S- Disulfide bond F(ab)’2 CH2 CH3 Figure 1 Diagram of a prototypic immunoglobulin (Ig)G monomer. Each rectangle represents an immunoglobulin domain. The complementaritydetermining regions (CDRs) are highlighted. The green bars depict the hinge region. The Fc fragment (for ‘fragment crystalline’) is produced by papain digestion of IgG and can be crystallized from a solution. It is the anchoring site for proteins of the complement system and for receptors of various effector cells. The Fab fragment (for ‘fragment antigen binding’) is produced by papain digestion of IgG. It possesses one combining site and can bind, but cannot precipitate, the antigen. The F(ab)’2 fragment is produced by pepsin digestion of IgG. Its molecular weight is double that of one Fab fragment, and it is capable of binding and precipitating the antigen. Antibodies 4 ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net
Antibodies ovided 0 that the ar he evidence that interactions are noncova supstance.called the adiuvant.that enhances immuno od that the antibodya genicity.Other factors that influence tions fron an lomain he dos the rout ids fr provide the specificity of the interaction.The shape and or particulate,native or denatured). size of the combining site can vary,depending on the complex analysed Pathogen neutralization 0 Antibodies recognize antigens outside the cells,where most pacteria and b erial toxins are for hey can bind to a over large sterically and electrostatically complementarity ock its Hydrophobic determinants of the antigen interac 地and bl nts anti an block the adhe of hact to hos cells by binding to cell-surface proteins used by the and r nd ac bacterium to adhere to the cell. rial adheren and prevent i hich ith Antibodies as a first line of defence IgM antibodies are generally encoded by germline Function ncoded variable region genes and are of low affinity However,be r pentameric stru n in th an bind tom resent on the ides Th xistence of 10antigen-binding sites on an IgM molec and enhances the strength of its effective binding. IgM and mp ow) also enective in comp he main i activation presented by MHC class U molecules to antigen cific T type i the ood and the helper cells.In tur,T lymphocytes provide signals for the tion and complement activation (see below).IgG anti differentia B cells. For the bodies can diffuse into tissues and bind toxins rapidly and m by with high aftinity a critical role in destruction of extracellular pathog gA he principal isotype in tions.particularly in prevention against the spread of intracellular infections and protection against toxins I he tw structural portions from infectious agents (Fc) ab constant against pathogen ted by the mot es col co Fab-mediated functions onferring nrote ection from infections to the newborn.The protective role of IgA is also indicated b Antigen recognition hat individuals deficient in A produce high The immune system is able to g nerate a large repertoire of titres of anti antibody-combining sites capable of recognizing virtually all possib presen pathogens and the nate in certain locations.For example.cholera toxins trigger few,if any,circulating antibodies but induce the production of IgA in the faeces of the infected individual carbohydrates,nucleic acids and phospholipids,but their Interaction with superantigens induction gener ation of the antigen a carner ENCYCLOPEDIA OF LIFE SCIENCES/e 2001 Nature Publishing Group /www.els.net
combining site, and have confirmed the domain-type organization of the immunoglobulin initially deduced from amino acid sequence analysis. They have provided evidence that antigen–antibody interactions are noncovalent and that the antibody-binding site requires contributions from the H- and L-chain variable domains of the Fab fragment. Amino acids from the CDRs, also called loops, between strands of the b sheets in the variable domains provide the specificity of the interaction. The shape and size of the combining site can vary, depending on the particular antigen–antibody complex analysed, from a shallow groove of 15 6 A˚ in size to a conical pocket of approximately 10 A˚ in diameter. The interactions occur over large sterically and electrostatically complementarity areas. Hydrophobic determinants of the antigen interact with hydrophobic determinants of the antibody-binding site, charged side-chains interact with side-chains of opposite charge, and proton donors and acceptors are involved in hydrogen bonding. Thus, electrostatic forces between charged amino acid side-chains (salt bridges), hydrogen bonds, van der Waals forces and hydrophobic forces, together with surface complementarity, impart antibody-specific recognition. Function For the immune system, immunoglobulins present on the B-lymphocyte surface play an essential role in transducing signals to the cytoplasmic and nuclear effectors, and in delivering the antigen to the cell compartment where it is degraded, processed and returned to the cell surface to be presented by MHCclass II molecules to antigen-specific T helper cells. In turn, T lymphocytes provide signals for the proliferation and differentiation of B cells. For the organism, antibodies secreted by B lymphocytes are responsible for the humoral immune response, which plays a critical role in destruction of extracellular pathogens, prevention against the spread of intracellular infections, and protection against toxins. The two structural portions of the antibody, i.e. the variable (Fab) and the constant (Fc) fragments, impart distinct biological functions. Fab-mediated functions Antigen recognition The immune system is able to generate a large repertoire of antibody-combining sites capable of recognizing virtually all possible antigens present in pathogens and their products (bacteria, viruses, and protozoal and metazoal parasites), and in environmental antigens. Antibodies can be raised against a variety of molecules, including carbohydrates, nucleic acids and phospholipids, but their induction generally requires the association of the antigen to a protein, called a carrier. Remarkably, even epitopes that do not exist in nature, such as synthetic haptens, can be exquisitely recognized and bound. A strong antibody response requires that the antigen be injected with a substance, called the adjuvant, that enhances immunogenicity. Other factors that influence immunogenicity include the dose injected, the route of immunization (subcutaneous injections are more immunogenic than intravenous injections) and the form of the antigen (soluble or particulate, native or denatured). Pathogen neutralization Antibodies recognize antigens outside the cells, where most bacteria and bacterial toxins are found. They can bind to a pathogen and block its access to cells, thereby preventing infection or destruction of host cells. In addition, antibodies can block the adherence of bacteria to host cells by binding to cell-surface proteins used by the bacterium to adhere to the cell. This binding will inhibit bacterial adherence and prevent infection. Antibodies may also be important for protection against viruses, which will not be able to infect cells and replicate. Antibodies as a first line of defence IgM antibodies are generally encoded by germlineencoded variable region genes and are of low affinity. However, because of their pentameric structure and their rapid generation in the blood, they can bind to multivalent antigens, such as bacterial cell wall polysaccharides. The existence of 10 antigen-binding sites on an IgM molecule enhances the strength of its effective binding. IgM antibodies are also effective in complement activation (see below). IgG is the main isotype in the blood and the extracellular fluid. It plays an important role in opsonization and complement activation (see below). IgG antibodies can diffuse into tissues and bind toxins rapidly and with high affinity. IgA is the principal isotype in secretions, particularly in the intestinal and respiratory tracts. Its main property is to neutralize foreign antigens and to protect epithelial cells from infectious agents, providing a first line of defence against pathogens. This is illustrated by the crucial role of sIgA present in the mother’s colostrum, which contains approximately the same level of sIgA as other external secretions, conferring protection from infections to the newborn. The protective role of IgA is also indicated by observations that individuals deficient in IgA produce high titres of antibodies against various milk antigens. In response to certain pathogens, IgA antibodies predominate in certain locations. For example, cholera toxins trigger few, if any, circulating antibodies but induce the production of IgA in the faeces of the infected individual. Interaction with superantigens Recently, a new type of lymphocyte ligand, referred to as a superantigen, has been discovered. Initially described for T Antibodies ENCYCLOPEDIA OF LIFE SCIENCES / & 2001 Nature Publishing Group / www.els.net 5